ی ا سعيذ علیه سی زاد جوطيذ هختاری 3- ػض يات ػ ی زوش تحميمات وطا رسی اتغ عثيؼی استا اس ذرا

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1 ی ا ششمین هما ی ش ملی ایده اهی نو رد کشاورزی تررسی خص صيات ر يطی اثرات ضذ قارچی ر ی تعذادی از 1 علی تر ا ی* قارچ صذفی قارچ ای تيواريسای گيا ی 3 2 سعيذ علیه سی زاد جوطيذ هختاری ت هی ج گل ای ضوال کط ر تر 1- ػض يات ػ ی زوش تحميمات وطا رسی اتغ عثيؼی استا اس ذرا 2- حمك در- ايست ا تحميمات ج زتغ پاس ذ 3- ػض يات ػ ی زوش تحميمات وطا رسی اتغ عثيؼی استا اس ذرا * ت ط ز غ ذ ق پستی 167 ايست ا تحميمات ج زتغ پاس ذ borhaniali@gmailcom چکيذ گ ای هختلف قارچ صذفی) spp ) Pleurotus تر ر ی گ ای هختلف درختی در قاط هختلف ج گل ای ضوال کط ر ر يص در ايي دار ذ Pleurotus ostreatus از جول فرا اى تريي آ است ک از جول قارچ تسيار خ راکی دار ئی تا ارزش هیتاضذ تررسی کطت خالص قارچ از طريق کطت تافت کال ک جوع آ ری ضذ از ج گل ای استاى ت ي ضذ کطت ا در ا ک تات ر تا دهای 22 درج سا تيگراد تذ ى ر گ ذاری ضذ ذ هيساى رضذ تط ر ر زا ا ذاز گيری ضذ سثت رضذ )GR( ضرية رضذ )GR( هحاسث ضذ خص صيات هيکر هرف ل شی هيسلي م ا تا ر ش کطت اساليذ تا استفاد هيکر سک ب ری ا جام ضذ کل ی قارچ سفيذ پ ث ای کوی اتتذا ترآهذ سپس صاف ضذ پطوی وذی هیگردد تراکن کل ی ارتفاع کل ی تترتية 1/2 2 ت د است هت سط سثت رضذ کل ی ا = 2/22 GR هيليوتر در ر ز تا ضرية رضذ = 21/22 GC پس از 13 ر زسطح پتری 9 سا تیهتری را پر کرد ت د تررسی اثرات ضذ قارچی ترر ی 2 گ قارچ تيواريسای گيا ی تا ر ش کطت د گا در هحيط کطت هالت اکسترات آگار ا جام ضذ تايج طاى داد ک ايي قارچ تر ر ی 3 گ از 2 گ قارچ ه رد استفاد اثر ک ترل ک ذگی داضت است تيطتريي اثر ک رل ک ذگی تر ر ی قارچ تيواريسای غالت sorokiniana هطا ذ ضذ است کليذ اش : قارچ ضذفی ج گل ای ضول کط ر, قارچ ای تيواريسای گيا ی هقذه : لارچ ای وال ىذار دست ای اس لارچ ا ست ذ و اس س ا ای تسيار د ر تطز تا آ آض ا ت د در ج ا غ خت ف تطزی ای خت ف آ تؼ ا غذا دار اد يز سا ر ا زدا ت شا رد استفاد لزار ی زفت ا ذ ساتم پز رش تى صی وطت تؼضی اس آ ا ت حذ د 2000 سا لث ی رسذ ا ز س طخع ضذ است و تيص اس 7000 لارچ تا درجات خت ف خ راوی دار ئی در د يا ج د دارد و 300 اس آ دارای ارسش خ راوی ػا ی ست ذ اس تي آ ا 200 در تاو ت ع ر ػ ػی وطت ضذ ا ذ 100 دارای ارسش التػادی ست ذ 60 در سغح تجاری 10 تغ ر غ ؼتی وطت ی ض ذ ت ع ريى شارا ت اسآ ت غ رت فزا رد ای غذائی لارچ غذفی يا Pleurotus ostreatus اس ج لارچ ای تسيار تا خت ف ت تاسار ای ج ا ی ػزض ی ض د( 1978 (Chang,Miles ارسش خ راوی دار ئی ی تاضذ و رد ت ج ج غ آ ری و ذ ا لارچ ای حطی ی تاضذ اي لارچ تػ رت ز ی ا ث ر ی ت ای افتاد يا س ذ ا اع خت ف در ختا ج ی ت ا ع سا تخػ ظ فػ تاتستا پائيش ر يص دارد تذ ي س ت وطت ت يذ

2 ي ششمین هما ی ش ملی ایده اهی نو رد کشاورزی در اغ ة ماط وط ر ت ت يذ آ ثادرت یض د ا زس فز ا ی ضذ اي لارچ را تز ر ی وا غالت وطت ید ذ يش اس خان ار خزد چ ب ای ای خت ف پ تزي ی ت ا تزای ت يذ استفاد د در اتغ ػ ی ت خ اظ خت ف دار ئی اي لارچ اضار ضذ است اس ج : تذ ي داضت اد Lavastatin تؼ ا يه دار يی ؤثز ج ت و تز وا ص و ستز خ و تز و ذ فطار خ وا ص د ذ غذ ای ت ر اضار ضذ يش دار يی م ی يز تخص ت ظي و ذ ر ای ل ة وارتزد دارد چ ي تذ ي پ ی ساواريذ ای ج د دارای آ تی تي تيىی تا خاغيت تاوتزی وطی آ تی يز س ی تاضذ ػػار تا ی جذا ضذ اس لارچ خاغيت ارو ذ ی (Iwalokun etal 2007, Sarangi ا ت اتات تج غ پالوت ای خ دارد( 2006 al et در اي تزرسی ض تزرسی تزخی اس خػ غيات ر يطی اي لارچ ت ا ىا استفاد اس اثزات ضذ لارچی اي لارچ تزای و تز تي صيه تي اري ای يا ی پزداخت ضذ است ه اد ر ش تحقيق : در اي تزرسی وطت خا ع لارچ غذفی تا استفاد اس وطت تافت وال ه لارچ ج غ آ ری ضذ اس ر ی ت درخت راش در غم ػثاس آتاد ت ط ز ت ي ضذ تزای اي ظ ر پس اس ضذ ػف ی سغحی وال ه لارچ تا اتا 66 درغذ لغؼاتی اس آ ا در 25 حيظ وطت ا ت اوستزان آ ار )MEA) لزار داد ضذ وطت ا در ا ى تات ر در تا د ای درج سا تي زاد تذ ر ) GR(4 ) GR( ذاری ضذ ذ شا رضذ تغ ر ر سا ا ذاس يزی ضذ سثت رضذ 3 ضزية رضذ تز اساس فز حاسث ضذ d = اي فز لغز و ی تز حسة ي ی تز در GC= dgh/t GR= Δd/Δt ت چا ) (Buchalo1988) =T ی تاضذ ريخت ض اسی تافت و ی تا استفاد وذ ا ضاخ ای رد تزاو و ی = g ارتفاع و ی =H س ا تز حسة ر س استفاد ت سظ استا پز ت )1978 ) Nobles1965,Stalper ت غيف ضذ ج د يا ػذ ج د ر ذا ا در حيظ وطت رد ارسياتی لزار زفت ت ظ ر تزرسی يىز سى پی س ي ای ر يطی لارچ پزپارسي س ي لارچ اس ر ش وطت اساليذ استفاد ضذ تزرسی خػ غيات يىز سى پی س ي ا تا استفاد يىز سى ج ا ي پ س BH2) (Olympus ج ش ت تزسي وا ثيز ضذ ا جا ضذ 5 لارچ تي اری سای يا ی ضا 3 لارچ پاساريت درختا ج ی: Ophiostoma ulmi Fusarium culmurum اس ر ی ضة خسة ت ر ش وطت تافت اس ر ی ج funerea Pestalotiopsis اس ر ی سى يا Fusarium oxysporum : چ ب درختا تي ار جذا ساسی ضذ ذ يش د لارچ تي اريشای غالت sorokiniana اس آس ايط ا لارچط اسی تي تى صی لارچ ا در دا طىذ تي صی دا ط ا ی ايز ا ت ي ضذ ارسياتی اثز ضذ لارچی لارچ غذفی تز ر ی لارچ ای تي اريشای يا ی تز ش وطت د ا در حيظ وطت ا ت اوستزان آ ار در 3 تىزار ا جا ضذ تزای اي ظ ر لغؼ ای تمغز 5 mm اس وطت تاس لارچ س ستا لغؼ ای ت ي ا ذاس اس لارچ تي اريشای يا ی ت فاغ 3cm اس ذي ز تز ر ی حيظ وطت Malt Extract Agar در پتزی د ای 25 درج سا تي زاد لزار داد ضذ ذ يشا ع اثز تمات تي ز د لارچ در 7 حا ت ى ت ر ش سيز رد ارسياتی لزار زفت ذ A=1 )ت لف رضذ ز د لارچ در ح تاللی( B=2) ت لف رضذ ز د لارچ تا فاغ اس ذي ز تذ تاللی(

3 C= 3 )رضذ يه لارچ تز ر ی دي زی تذ ت لف رضذ در ح تاللی( = CA1 5/3) رضذ سثی يه لارچ تز ر ی يه لارچ دي ز تؼذ اس ت لف ا ي رضذ در ح تاللی( =CA2 4) پ ضص وا يه لارچ تز ر ی يه لارچ دي ز تؼذ اس ت لف ا ي رضذ در ح تاللی ) = 5/4 )رضذ سثی يه لارچ تز ر ی يه لارچ دي زتؼذ اس ت لف رضذ ز د لارچ تا فاغ اس ذي ز( CB1 =CB2 5 )پ ضص وا يه لارچ تز ر ی يه لارچ دي زتؼذ اس ت لف رضذ ز د لارچ تا فاغ اس ذي ز تيج تحث: يا لارچ غذفی: اس لارچ ای خ راوی تا ارسش دار ئی ی تاضذ و در اغ ة فػ سا ی ت ا ذ Pleurotus ostreatus يافت ض د لارچ دارای وال ىی ت لغز 14 6 سا تي تز سال ای ت ت ذی 4 2 سا تي تز لغزی ت ا ذاس 1 3 سا تي تز است لارچ ت ضى غذفی تا ر ای ت ع ت د اس ج ل ای يا آتی خاوستزی تيز سپس ت ر خاوستزی ل ای ی تاضذ دارای تافتی سفيذ ر ی تاضذ اسپ ر ا سفيذ تا خاوستزی ت فص و ز تيض ی ت ا ذاس /5 يىز است لارچ ذت ر تػ رت ز ی ا ث ر ی ت ای افتاد يا س ذ درختا راش, افزا ت ط حتی س س ی تز ا در ارتفاع در ت ا ع سا تخػ ظ فػ تاتستا پائيش ديذ ضذ است و ی لارچ سفيذ پ ث ای و ی اتتذا تزآ ذ سپس غاف ضذ پط ی ذی ی زدد تزاو و ی ارتفاع و ی تتزتية 1/5 2 ت د = GC پس اس 13 GR = ت سظ سثت رضذ و ی ا 6/62 ي ي تز در ر س تا ضزية رضذ 21/56 ر سسغح پتزی 6 است 6-3 يىز سا تی تزی را پز وزد ت د خػ غيات يىز زف صی يس ي ا طا داد و لغز يف تا يز ای اتػا فزا ا تمزيثا در دي ار ای ػزضی در ا ذاس ای 7 4 تا 3 2/5 يىز تزرسی اثزات ضذ لارچی تزر ی 5 لارچ تي اريشای يا ی تا ر ش وطت د ا در حيظ وطت ا ت اوستزات آ ار ا جا اس 5 ضذ تايج طا داد و اي لارچ تز 3 ر ی لارچ رد استفاد اثز آ تا يستی داضت است تيطتزي ضاخع آ تا يستی تز ر ی لارچ تي اريشای غالت sorokiniana طا ذ ضذ است لارچ ای Ophiostoma ulmi Pestalotiopsis funerea ػا زي يز س س یتز ا در درجات تؼذی لزار داضت ذ ػا زي ار Pleurotus ostreatus تز ر ی 3 اس 5 تزرسی اثز ضذ لارچی در جذ ض ار )1( آ ذ است طا ی د ذ و لارچ لارچ رد استفاد اثز آ تا يستی داضت است تيطتزي اثز آ تا يستی تز ر ی لارچ تي اريشای غالت تا ع او ص C ز B2 آ تا يستی 5/4 طا ذ ضذ است لارچ ای Ophiostomaػا ulmi زي sorokiniana ار تا ع او ص C A2 ز آ تا يستی 4 A زي يز س س ی تز ا تا ع او ص Pestalotiopsisػا funerea F oxysporum F culmurum ز آ تا يستی 1 در درجات تؼذی لزار داضت ذ در مات تز ر ی لارچ ای اثز آ تا يستی ذاضت است

4 جذ ض ار )1( ز ا تا يستی ع ػىس ا ؼ رلاتتی تي لارچ غذفی لارچ ای تي اريشای يا ی Plant pathogenic fungi Agent of disease Type of reaction Antagonistic Score Fusarium oxysporum Wilt 0 گيری diseases of silk tree Ophiostoma ulmi Dutch elm C A2 4 disease of تيج کلی: تايج elm trees ت ج تا آ ذ لارچ Pestalotiopsis Fusarium culmurum Die back of Foot rot of A C A2 * 1 0 funerea conifers cereal تذست P C B1 45 sorokiniana Foot root of cereal C A1 * sorokiniana, Ophiostoma ulmi, Pestalotiopsis ت ا ائی و تز لارچ ای تي اريشای ostreatus funerea در ضزايظ حيظ وطت را دارد ت اتزاي تزرسی ا ىا استفاد وارتزدی اس اي لارچ تخػ ظ تزای و تز تي اری زي ار و يىی تزي تي اری ای ای خت ف درختا خا دا ار در ج ای ض ا وط ر ی تاضذ تايستی اس ا ذاف غا ؼات تؼذی تاضذ ه اتع: 1- Buchalo AS 1988 The higher edible Basidiomycets in pure culture Nauk Dumaka press, kiev,143pp 2- Chang S T, Miles PG1978 Historical record of the early cultivation of Lentinus in china,mushrooms J Tropics Iwalokun, B A1, Usen, U A1, Otunba, A A2 and Olukoya, D K2 (2007) Comparative phytochemical evaluation, antimicrobial and antioxidant properties of Pleurotus ostreatus African Journal of Biotechnology 6 (15), pp , 4- Sarangi Itisam, Bhutia, Sujit, Mallick, Sanjaya, Maiti, Tapas(2006) Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans International Immunopharmacology 6,( 8)

5 Stalpers JA, 1978 Identification of wood inhibition Aphyllophorales in pour cultures Stud Mycol16: 248P Study on growth characteristics and possibility of usage Pleurotus ostreatus the native mushrooms of northern forests on contores of some plant platogenic fungi Ali Borhani* 1,,Saied Ali Mosazadeh 2 Jamshid mokhtari 1- Agriculture and Natural Research Center of Mazandaran Passand forest and rangeland research station PO Box 167 Behshar- Iran borhaniali@gmailcom 2- researcher on Passand forest and rangeland research station, 3- Agriculture and Natural Research Center of Mazandaran *Passand forest and rangeland research station PO Box 167 Behshar- Iran borhaniali@gmailcom Abstract: Pleurotus spp grow on different on different tree species on northern forest of Iran, and Pleurotus ostreatus is more common than others, is one of the most valuable medicinal and edible mushroom For this study Pour culture of this mushroom achieved by tissue culture of fruiting body which collected from Mazandaran forest areas they were incubated in 25 oc darkness Daily growth was measured, growth rate and growth coefficient were calculated Mat: white, slightly raised cottony on newest growth, then collapsed, wooly felty, with slightly raised tufts arranged The density of the colony and the height of the colony were 2 and 15mm respectively The average of growth rate was 662 mm/day and growth coefficient was 2156 Plates were covered by 13 days Hyphal width: 3-9 µm Clamp connection present in almost all of septa in different size and shapes µm, Antifungal activities experiment has been done against 5 plant pathogenic fungi in dual culture on MEA and 3 replications The result showed this mushrooms controlled 3 of 5 plant pathogenic fungi,the most have been seen the agent of cereal kernel blight fungi sorokiniana Key word :, Pleurotus ostreatus, northen forest of Iran, plant-pathogenic fungi

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